Study: male infertility and the microbiota of the seed fluid
Study: male infertility and the microbiota of the seed fluid
Reference
Weng SL, Chiu cm, lin fm, et al. Bacterial communities in the seeds of men of sterile couples: metagenomic sequencing reveal relationships between seed microbiota and seed quality. Plus one. 2014; 9 (10): E110152.
Design
retrospective cohort of seed samples that are subjected to bioinformatic analysis
participant
in the reproduction center of the Mackay Memorial Hospital, Taipei, Taiwan, Republic of China, 96 seed samples were taken from men, in which primary infertility has been diagnosed for at least one year. Sama samples of 36 fertile men were also included in the study. All participants were between 26 and 58 years old and had no other significant health problems.
Study intervention
seed samples were collected, analyzed on bacterial communities and rated seed quality by measuring seed volume, sperm concentration, strict morphology, atypical forms and leukocytes. A computer -aided semen analysis (CASA) was also carried out. The seed was collected in a sterile mug after 3 to 5 days of abstinence.
important knowledge
of the 96 samples The most common flora was found in the seed fluids lactobacilli (19.9 %), pseudomonas (9.85 %), Prevotella (8.51 %) and Gardnerella ). It was discovered that bacterial communities are grouped in the sperm in 3 main groups (G1-G3). G1 was characterized by the maternity genre pseudomonas and contained bacteria from the bazillus , pelomonas , rhondanobacter , Enterobacter , Klebsiella , brevibacillus Orchrobaktrum genera. G2 was characterized by the mother genre lactobacilli and contained bacteria from the Enterobacter Klebsiella, pseudomonas stenotrophomonas , bazillus , chrysobacterium brevibacillus and ochrobaktrum genera. G3 was characterized by the maternity genre Prevotella and also contain bacteria from the pedobacter , propionibacterium , dietzia and halomonas .
Normal samples pointed out significantly higher values on lactobacilli and Gardnerella during samples that were classified as of low quality, Prevotella . It is noteworthy that 80.6 % of the normal seed samples in the clusted lactobacilli group (G2). The comparative analysis shows that the samples in the G1 and G3 groups had a 5.2-fold and 8.5x higher probability of containing two abnormal seed parameters compared to the samples in the G2 group. According to Casa, morphology was the only seed parameter that was strongly associated with pathogenic bacteria.
practice implications
infertility or the absence of a conception after 12 months of unprotected sexual intercourse concerns 15 % of all couples, and almost 50 % of these cases are attributed to male infertility. There is indications that male fertility has decreased by 2 % per year in the past decades, with an estimated decline in the mean sperm number of around 0.94 x 106 per year. Play male infertility. Infections of the prostate, secondary dogs and testicles play a causal role in 15 % of male infertility. 3.4 seed bacteria and leukocytes affect fertility by affecting spermatogenesis and reducing sperm function.
The authors of this study come to the conclusion that, based on their results, they believe that supplementation with lactobacilli could help support a healthy microbiome in the seed fluids and thereby maximize the fertility potential.
The composition of the human microbiome affects health and can contribute to diseases. 5,6 A previous study by Ibadin and Ihle with the use of the cultural method showed that 41.4 % of the infertile men had at least 1 of the following bacteria: Staphylococcus aureus (16.9 %)), Staphylococcus saprophytic (9.2 %), Escherichia coli (6.9 %), Proteus mirabilis (3,4 %), Klebs SPP (2.3 %), pseudomonas aerocuginosa Proteus vulgaris (2.3 %). They were identified in seed samples with abnormal overall motility and morphology. carried out cultural studies on 696 seed samples and found that the sperm concentration, mobility and morphology in the presence of most likely abnormally Gardnerella vaginalis and ureaplasma urealyticum . Use of cultural or polymerase chain reaction-based methods.
The authors of this study aim to improve the understanding of bacterial seed communities by presenting an analysis flow using the next generation sequencing technology in connection with clinical seminal analysis. The most common genres found in this study support the results of Hou et al., Which also support seed microbiota in fertile and sterile men with another method of pyrosequencing bacterial ribosomal ribonucle acid. lactobacilli could help support a healthy microbiome in the seed fluids and thereby maximize the fertility potential. Larger follow -up studies are justified to confirm this opinion and further clarify the mechanism, cause infertility by pathogenic bacteria.
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