Effects of amino acid mixture on cortisol levels induced by physical exertion

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Bezug Tsuda Y, Murakami R, Yamaguchi M, Seki T. Akute Supplementierung mit einer Aminosäuremischung unterdrückte die durch körperliche Betätigung induzierte Cortisolreaktion bei in der Freizeit aktiven gesunden Freiwilligen: eine randomisierte, doppelblinde, placebokontrollierte Crossover-Studie. J Int Soc Sports Nutr. 2020;17(1):39. Studienziel Es sollten die Auswirkungen einer akuten Einzeldosis einer Aminosäuremischung, die Arginin, Valin und Serin enthält, auf männliche Teilnehmer mit einer nachgewiesenen hohen Cortisolreaktion auf körperliche Betätigung bestimmt werden Entwurf Randomisierte, doppelblinde, placebokontrollierte Crossover-Studie Intervention Kapseln mit entweder 1,8 g Arginin, 1,1 g Valin und 0,1 g Serin oder Placebo (leere Kapseln) 30 Minuten bevor die Teilnehmer einen Belastungsversuch auf einem …
Cover Tsuda Y, Murakami R, Yamaguchi M, Seki T. Acute supplementation with amino acid mixture suppressed the cortisol reaction induced by physical activity in the healthy volunteers: a randomized, double-blind, placebo-controlled crossover study. J inte at soc sports nutr. 2020; 17 (1): 39. The aim should be the effects of an acute single dose of an amino acid mixture that contains arginine, Valin and Serin, to male participants with a proven high cortisol reaction to physical activities, draft, double blind, placebokontrolled crossover-study intervention capsules with either 1.8 g arginine, 1.1 g Valin and 0.1 g Serin or placebo (empty capsules) 30 minutes before the participants have an attempt to stress on a ... (Symbolbild/natur.wiki)

reference

tsuda y, Murakami R, Yamaguchi M, Seki T. Acute supplementation with an amino acid mixture suppressed the cortisol reaction induced by physical activity in healthy volunteers: a randomized, double-blind, placebo-controlled crossover study. j int -soc sports nutr . 2020; 17 (1): 39.

Study goal

The effects of an acute single dose of amino acid mixture that contains arginine, Valin and Serin should be determined on male participants with a proven high cortisol reaction on physical activity

draft

randomized, double blind, placebo-controlled crossover study

Intervention

Capsules with either 1.8 g arginine, 1.1 g Valin and 0.1 g Serin or placebo (empty capsules) 30 minutes before the participants performed a stress on a bicycle ergometer (aerobics 75xliii) at 50 % VO 2

To prevent an drying out, the test subjects drank the same amounts of water during a 3-minute break.

In the opinion of the study authors, indirect effects of the amino acids could explain the decrease in the training -induced cortisol.

After a one -week outwatering phase, the participants returned to change the study on the other arm.

The researchers collected blood samples from the brachial vein immediately before and after training.

participant

Twenty "leisure-active" men between the ages of 20 and 39 (mean 32.3 ± 1.2 years), medium body mass index (BMI) 22.3 ± 0.4. After the removal of 5 participants due to abnormal blood analyzes or protocol deviations, 15 participants contributed to the final analysis.

study parameters evaluated

  • plasma navy (MCG/DL)
  • adrenocorticotocropes hormone (acth, pg/ml)
  • cortisol/acth ratio
  • blood sugar (mg/dl)
  • plasmalactat (mg/dl)
  • Plasma-Amiak (McG/DL)
  • Serum-creatine phosphokinase (CPK, U/L)
  • total ketone body in the serum (µmol/l)
  • Free fatty acids in the serum (meq/l)

primary result measurements

Changes to the plasmacortisol concentration in the blood within each group (intervention and placebo) and between the groups

important knowledge

internal results:

cortisol: In the placebo group, the plasma cortisol was significantly higher after training than the cortisol before training (9.51 ± 0.85 compared to 14.39 ± 2.15, p <0.05), while there was no significant difference in the treatment group (9.71 ± 0.99 ± 1,23, p = 0.846).

acth: In the placebo group, the plasma act was significantly after training (24.21 ± 2.91 vs. 53.17 ± 6.97, p <0.01), while the change in the treatment group was not significant (27.33 ± 3.60 compared to 46,92 ± 10.41, p = 0.057).

cortisol/acth ratio: The participants in both the placebo and the treatment group showed a significant increase in cortisol/acth relationship after training compared to before training ( p <0.01).

Intergroup results:

cortisol: The increase in plasmacortisol before and after the training was significantly lower in the treatment group compared to placebo (0.28 [−2.75, 3.31] compared to 4.87 [0.89, 8.86], p <0.05).

acth: There was no significant difference between the 2 groups for the changes in the plasma-acth during training (28.96 [13.5, 44.4] for the placebo group vs. 19.59 [−0.7, 39.8] for the treatment group, p = 0.454).

cortisol/acth ratio: The changes in the cortisol/acth ratio before and after the training were not significantly different between the two groups.

Blutzucker, plasmalact deed, plasmaamammoniak, serum CPK, total ketone body in the serum and free fatty acids in the serum, all after training, changed significantly compared to before training ( p <0.01) within each group. All of these analytes increased significantly after training, with the exception of the blood sugar, which dropped significantly in both groups ( p <0.01).

When comparing the amino acid intervention group with placebo, however, there was no significant differences between the groups for any of the analytes mentioned above.

practice implications

cortisol is released in the event of intensive physical activity in response to the drop in blood sugar. The physiological effect of cortisol is to maintain circulating glucose by increasing glycogen breakdown (glycogenolysis) in muscles and liver. This is generally due to an increased secretion of ACTH from the pituitary gland, which stimulates the release of cortisol from the adrenal gland.

Interestingly, the plasmcortisol anchor after training in the amino acid group was weakened compared to placebo, although there was no significant difference in the ACTH between the two groups. Since the ACTH has not been significantly reduced by taking the amino acid mixture, the mechanism of action that leads to the oppressed cortisol response is unclear.

In the opinion of the study authors, indirect effects of the amino acids could explain the decrease in the training -induced cortisol. Arginine promotes fat metabolism, 1.2 What can help to maintain the glycogen or glucose level in the blood. It has been shown that Valin (and leucine, but not isolucin) reduces the stress -induced cortisol an increase in rats. 3 and Serin can increase the production of phosphatidylserine, which was shown in a clinical study that it is reduced by physical activity.

While there were a statistically significant reduction in the cortisol after training for those who took the amino acid combination, the clinical relevance is unclear. The researchers did not test 2 max, fatigue, perceived exertion or recreational time. This would have provided additional data to understand whether the biochemical changes are also reflected in changes in performance.

An earlier study carried out by the same researchers evaluated the chronic intake (14 days) of the same combination of amino acids. They then trained by bike, and as with the current study, the measurements were carried out after the training. The subjective assessment of fatigue based on a visual analog scale (VAS) and an evaluation of the perceived effort (RPE) significantly improved compared to placebo. In addition, the climbs of the total ketone body in serum during training and the tryptophan/branched chain amino acids (BCAA) were significantly lower in the amino acid group compared to placebo. This implies that long-term and acute dose of amino acids probably have different effects.

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